INTRODUCTION:

Since ages, Indian people had an incredible knowledge of phyto-medicine driven apparently by a tremendous passion for the study of medicinal plants. The earliest description of the medicinal plants and their uses is to be found in Rig Veda - the oldest scripture in the world. Probably no other medicinal culture in the world has so extensive, detailed and deep an understanding about the medicinal value of plants. Most of the medicinal systems, including Greek and Roman have freely borrowed from Ayurveda. The entire world has now realized that, being natural, the Indian medical preparations are not only harmless, vital, vigorating and life-giving, but are within the reach of common man.

This research article has captures the very important part of phytochemical investigation^1,2 and characterization of constituent which is obtained from Syzygium cuminii (L) skeel³.

METHODOLOGY:

Plant materials

Eugenia jambolana (Syn.Syzygium cuminii (L) Skeel ), belonging to the Family: Myretacea Commonly known as ‘’Jamun’’ . Roots were collected during the month of April 2009 from village Nalwar of Gulbarga District (Karnataka) and were identified and authenticated by Department of Botany, Gulbarga University Gulbarga, a voucher (#72) of specimen was submitted to NGSMIPS, Derlakatte.

Preparation of extract:⁴

The powder plant material was successively extracted with petroleum ether, ethyl acetate and ethanol in a soxhlet apparatus for 72 hrs, the marc left behind was cold macerated with double distilled water. All extracts were filtered through Whatmann paper and concentrated by vacuum evaporation. The preliminary phytochemical screening of different extracts showed the presence of carbohydrate, flavonoids and terpenoids. The dried extracts were suspended in 2% gum acacia solution and used for animal experiments⁵.

The residue (25 g) was triturated in mortar with CHCl₃ (10 ml) and adsorbed onto silica gel (20g). After evaporation of the solvent it was loaded onto a silica gel column (150 g) prepared in petroleum ether (60-80˚C). ). The column was eluted with the graded (difference of 10) solvents according to the increasing polarity of the solvents pet.ether. benzene, chloroform, methanol. The component which is obtained in the ratio of chloroform: methanol elution was designated as ScRex-6b as pure compound which was 2.5gm so plane was made to prepare a gel to find out the analgesic activity ( the detail regarding pharmaceutical parameter in the paper 2 of same magazine) it also gave +ve test for flavon glycosides(Shinoda test .Zn-HCl reduction test)

3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-1-benzopyrone-4-one.gentobios

Melting point: 350ºC; Rf value 0.42 (solvent1692cm^-1system; C₆H₆: EtOAc (80:20);IR bands (KBr): 3430, 2928,and1692cm^-1.HNMR (CDCL₃) : δ 0.5- δ 3.9 (number of CH, CH₂ protons); MS m/z (rel.int.) 663: [M]⁺ (C₂₇H₃₅O₁₉),663, 453(90%), 407 (60%), 284 (10%), 248 (70%).

Acute toxicity studies⁵

The acute toxicity studyof chloroform extract was carried out in adult female albino rats by “up and down” method (OECD guidelines 425). The animals were fasted overnight and next day chloroform extract of the root Syzygium cuminii (L) skeel dissolved in 0.6 % sodium CMC was administered orally at different dose levels. Then the animals were observed continuously for three hours for general behavior, neurological and autonomic profiles. The observations are tabulated according to ‘Irwin’s Table’. The extract showed no toxic effects. This may be attributed to the fact that the root Syzygium cuminii (L) skeel has the food value.

DISCUSSION AND CONCLUSION:

In the present work we have isolated the single compound from the mentioned title plant from ethanolic extract. The isolated phytoconstituents are subjected to phytochemical study characterization and interpretation, then we have concluded that the present phytoconstituents are Myristine gentobioes, more ever the isolated constituent where subjected to acute toxicity studies , The observations are tabulated according to ‘Irwin’s Table’. The extract showed no toxic effects. This may be attributed to the fact that the root Syzygium cuminii (L) skeel has the food value. The forther work on screening for pharmacological activity and pharmaceutical work is caring out in a college lab

REFERENCE:

1. Sagarwat H, Mann AS, Khare MD, pharmacological potential of Eugenia Jambolana.A review.PHCOG MAG;2006:96-105

2. Kirtikar KR, Basu BD, Indian medicinal plant .vol.II, Delhi. Periodical express; 1975:1052-53.

3. Bhattacharejee SK, Hand book of aromatic plant. Pointer publisher; 1999:195-197.

4. Nadakarni KM, Indian Mataria Medica. Vol.I, popular book deportment Bombay; 1954:516-18.

5. New OECD 425 guidelines for testing of animal; 2001dec:1/26,1-6.

Received on 15.12.2010 Modified on 12.01.2011

Asian J. Research Chem. 4(5): May, 2011; Page 800-801