Table: -1: - Composition and Characteristics of Lavender Essential Oil Components ^22-25

Sr. No	Component	Percentage (%)	Characteristics
1	Linalool	20-45%	Floral, sweet, woody aroma; calming, relaxing effects
2	Linalyl Acetate	25-50%	Sweet, fruity scent; sedative, anti-inflammatory properties
3	Camphor	0.1-1.5%	Woody, camphoraceous note; stimulates circulation
4	Terpinen-4-ol	2-6%	Herbal aroma; antimicrobial, anti-inflammatory properties
5	Lavandulyl Acetate	2-6%	Sweet, fruity, floral aroma; relaxing, sedative effects
6	β-Caryophyllene	2-5%	Spicy, woody aroma; anti-inflammatory, analgesic properties
7	1,8-Cineole (Eucalyptol)	0.1-2%	Fresh, camphoraceous note; expectorant, antimicrobial
8	α-Pinene	0.1-1.5%	Pine-like aroma; bronchodilator, anti-inflammatory properties
9	Geraniol	0.1-1%	Sweet, floral scent; antioxidant, antimicrobial properties
10	Borneol	0.1-1.5%	Woody, camphoraceous note; enhances circulation, antimicrobial properties
11	Lavandulol	0.1-1%	Floral, woody aroma; contributes to the overall fragrance profile
12	Myrcene	0.1-1%	Earthy, fruity aroma; analgesic, sedative properties
13	Ocimene	0.1-1%	Sweet, herbal scent; contributes to the overall fragrance profile
14	Camphene	0.1-0.5%	Woody, herbal aroma; antioxidant, antimicrobial properties

Table 2: Chemical composition of lavender oil ^22-25

Sr. No	Name	Chemical Formula	Molecular Weight (g/mol)	Structure
1	Linalool	C₁₀H₁₈O	154.25
2	Linalyl Acetate	C₁₂H₂₀O₂	196.29
3	Camphor	C₁₀H₁₆O	152.23
4	Terpinen-4-ol	C₁₀H₁₈O	154.25
5	Lavandulyl Acetate	C₁₂H₂₀O₂	196.29
6	1,8-Cineole (Eucalyptol)	C₁₀H₁₈O	154.25
7	α-Pinene	C₁₀H₁₆	136.24
8	Geraniol	C₁₀H₁₈O	154.25
9	Lavandulol	C₁₀H₁₈O	154.25
10	Myrcene	C₁₀H₁₆	136.24

2. EXTRACTION METHOD:

2.1 Steam Distillation:

The steam distillation is oldest method to extracted the lavender oil from dry flower. In this method is very famous in industry due requirement of low investment cost on instrument and simple process.in this method the steam is Pass through the flower to separate the oil from flower. The steam distillation is energy intensive process, The typical yield of lavender oil from steam distillation is about 1-3% of the dry weight of the plant material used.^26-27.

2.2 Hydro Distillation:

The hydro distillation is also one of the old distillation methods, it is used to replace the Steam distillation. it produce the good quality of oil than the steam distillation. In this method the lavender flower is soak into water then heat the system and collect the distillated into receiver The yield of lavender oil obtained through hydro distillation typically ranges from 0.2% to 1.5% of the dry weight of the lavender flowers used. Compared to steam distillation, hydro distillation generally produces lower yields of essential oil.^26-28.

2.3 Solvent Extraction:

The Solvent extraction is method in which lavender oil is extracted from the flower. The yield of lavender oil obtained through solvent extraction can be higher than that obtained through steam or hydro distillation. However, the process also has some drawbacks, including the use of solvents, which can be hazardous if not handled properly, and the possibility of residual solvent remaining in the final product. In addition to the yield, the quality of the essential oil obtained through solvent extraction can also vary depending on factors such as the type and quality of the solvent used, for this research is used the solvent like hexane, get the better yield and good quality of oils^28-29.

2.4 Supercritical Fluid Extraction:

The realm of analytical applications has been revolutionized by the emergence of Supercritical Fluid Extraction, a technique that has garnered significant attention in recent times. At the forefront of this innovation is Supercritical CO2 extraction, heralded for its eco-conscious approach and enhanced efficiency in harvesting essential oils. This method stands out for its ability to sidestep the use of traditional solvents, while also offering the added benefit of CO2 recyclability. [30-31One of the hallmarks of this technique is its capacity to yield a substantial amount of essential oil with exceptional purity. However, like any advanced technology, it comes with its own set of challenges. The process demands a higher financial investment compared to conventional methods, and success hinges on meticulous control of various parameters including temperature and pressure. This precision is crucial to ensure the extracted essential oils meet high-quality standards and remain free from unwanted substances. In the specific case of lavender oil extraction, the yield obtained through supercritical CO2 extraction showcases remarkable variability. Scientific investigations have documented yields spanning from a modest 0.3% to an impressive 4.5% of the dry weight of lavender flowers used. This wide range is attributed to a complex interplay of factors, including the specific pressure and temperature conditions employed, the rate at which CO2 flows through the system, and the overall duration of the extraction process.^32-33

2.5 Microwave-assisted extraction:

Harnessing electromagnetic waves, microwave-assisted extraction emerges as a swift and potent technique for drawing out the essence of plants. This method's rapid heating and abbreviated processing time work in tandem to safeguard the delicate aromatic compounds and chemical integrity of essential oils. However, this advanced approach comes with its own set of challenges, including higher costs and the need for specialized equipment, setting it apart from more traditional methods like steam or hydro distillation.^34-35. When it comes to lavender oil specifically, the bounty reaped through microwave-assisted extraction is not a fixed quantity. Instead, it fluctuates based on a constellation of variables. These include the calibre and volume of lavender blossoms used, the nature and concentration of the chosen solvent, and the duration of the extraction process itself. Scientific investigations have unveiled a spectrum of yields, with lavender oil production ranging from a modest 0.6% to a more substantial 2.5% of the dry mass of lavender flowers subjected to this extraction method.^36-38

2.6 Ultrasound-assisted extraction:

Ultrasound-assisted extraction (UAE) harnesses the power of high-frequency sound waves to disrupt plant cellular structures, liberating essential oils from their botanical confines. This innovative technique has proven particularly effective in the extraction of lavender oil, offering a blend of efficiency and efficacy. The yield of lavender oil obtained through UAE exhibits considerable variability, with scientific studies reporting a range from 0.5% to 3.5% of the dry weight of lavender flowers utilized. This spectrum reflects the method's potential to extract significant quantities of the prized oil. When compared to conventional extraction techniques such as steam distillation and solvent extraction, UAE boasts several distinct advantages. It stands out as a swift and efficient process, significantly reducing both the time required and the volume of solvent needed. Moreover, UAE often results in higher essential oil yields, maximizing the return on raw materials. Perhaps most intriguingly, UAE has demonstrated the ability to produce essential oils with enhanced chemical complexity and more nuanced aroma profiles. This suggests that the oils extracted via this method may more faithfully represent the full spectrum of volatile compounds present in the original plant material.^39-41.

2.7 Enzymatic extraction:

Enzymatic extraction represents a novel approach in the realm of essential oil procurement, leveraging the catalytic power of enzymes to dismantle plant cell walls and liberate aromatic compounds. This method specifically targets cellulose and hemicellulose, key structural components of plant cells, to facilitate oil release. In the context of lavender oil extraction, this technique has shown considerable promise. Scientific investigations into enzymatic extraction of lavender oil have revealed a yield spectrum ranging from 0.5% to 3.5% of the dry weight of lavender flowers used. ¹¹ This range suggests that the method can be quite effective in extracting significant quantities of the desired oil. When juxtaposed with traditional extraction methods like steam distillation and solvent extraction, enzymatic extraction offers several distinct advantages. Perhaps most notably, it's characterized by its gentle nature. This softness in approach helps preserve the intricate chemical composition and delicate aroma profile of the essential oil, potentially resulting in a final product that more closely mirrors the natural bouquet of the lavender plant. Furthermore, enzymatic extraction aligns well with growing environmental concerns. By utilizing natural enzymes, this method reduces the need for harsh chemical solvents and high-temperature processes. This not only minimizes the environmental footprint of the extraction process but also aligns with consumer preferences for more natural and eco-friendly production method.^41-43

2.8 Micellar extraction:

Micellar extraction represents an innovative approach in the realm of essential oil procurement, harnessing the unique properties of surfactants to enhance the extraction process. This technique revolves around the formation of micelles - microscopic spherical structures created by surfactant molecules in solution. These micelles act as molecular encapsulates, effectively solubilizing essential oil compounds and facilitating their extraction from plant material. In the specific case of lavender oil, micellar extraction has demonstrated impressive efficacy. Scientific studies have reported yields ranging from 1.5% to 4.5% of the dry weight of lavender flowers used. This substantial range underscores the method's potential for extracting significant quantities of the prized oil.²⁷ When compared to traditional extraction methods such as steam distillation and solvent extraction, micellar extraction offers a suite of advantages. Its relative simplicity and cost-effectiveness make it an attractive option for both small-scale and industrial applications. The method typically requires less time and solvent, streamlining the production process and potentially reducing operational costs. Perhaps most intriguingly, micellar extraction has shown the capacity to yield essential oils with enhanced chemical complexity and more nuanced aroma profiles. This suggests that the oils extracted via this method may more faithfully represent the full spectrum of volatile compounds present in the original plant material, potentially resulting in a final product of superior quality and authenticity.^39-44

3. Application of Lavender oil:

3.1 Cosmetic, Personal and Home care Industries.

The aromatic allure of essential oils has long been a cornerstone in the cosmetics industry, captivating consumers for generations. However, recent scientific inquiries have unveiled a more profound potential for these botanical extracts, particularly in the case of lavender essential oil. This fragrant elixir is now emerging as a multifaceted asset in the realm of cosmetics and personal care. At the forefront of this revelation is lavender oil's prowess as a natural preservative. This discovery comes at a crucial juncture, as the nutrient-dense formulations of modern cosmetics create an ideal breeding ground for potentially harmful microorganisms. While chemical preservatives have traditionally been the guardians of product integrity, their use has increasingly come under scrutiny due to potential adverse health effects. Enter lavender essential oil - a promising natural alternative that may help mitigate the need for synthetic preservatives. ^45-47. The antimicrobial properties of lavender oil extend beyond mere preservation. Studies have demonstrated its efficacy in combating oral malodour, suggesting potential applications in oral care products. Furthermore, its preservative effects have been observed in a diverse range of products, from washing liquids and soft body balms to aqueous creams, highlighting its versatility in formulation. This shift towards natural alternatives resonates with a growing consumer consciousness in the home care industry as well. The demand for lavender-scented products has seen a notable uptick, with its fragrance finding its way into everyday household items like detergents and floor cleaners. This trend underscores a broader movement towards natural fragrances in consumer goods. In essence, lavender essential oil is carving out a new niche for itself in the world of cosmetics and personal care. It stands not just as a pleasant scent, but as a functional ingredient with the potential to enhance product safety and align with evolving consumer preferences for natural alternatives. ^46-49

3.2 Applications of Lavender Essential Oils in Aromatherapy:

Lavender essential oil stands as a cornerstone in the realm of aromatherapy, a practice that harnesses the power of plant essences to nurture holistic wellness. This fragrant elixir, extracted from the delicate purple blooms of the lavender plant, has earned its reputation as a versatile remedy for a myriad of physical and psychological concerns. At the heart of lavender oil's appeal lies its remarkable ability to soothe and calm. When diffused into the air or added to a warm bath, its gentle aroma creates an atmosphere of tranquillity, helping to melt away the day's stresses and anxieties. For those grappling with the restlessness of insomnia, lavender's soporific qualities offer a natural pathway to restorative sleep. Beyond its role as a balm for the mind, lavender oil showcases impressive analgesic and anti-inflammatory properties. This makes it a valued ally for those battling chronic pain, persistent headaches, or debilitating migraines. When applied topically, it works to quell inflammation and provide welcome relief from discomfort.^50-52 In the domain of skincare, lavender oil emerges as a multi-faceted remedy. Its natural antibacterial and antifungal attributes make it an effective treatment for various skin ailments, from stubborn acne to persistent eczema. Moreover, it holds the potential to diminish the visibility of scars and wrinkles, contributing to an overall improvement in skin tone and texture. While lavender oil's therapeutic potential is vast, it is crucial to approach its use with mindfulness and respect. As with all potent natural remedies, it is advisable to seek guidance from a qualified aromatherapist or healthcare professional to ensure safe and effective application. In essence, lavender essential oil embodies the harmonious fusion of nature's bounty with therapeutic intent. Its ability to address a spectrum of physical and emotional needs underscores its status as a true aromatherapeutic powerhouse. ^51-53

3.3 Lavender Essential Oils as Natural Preservatives:

Lavender essential oil, renowned for its enchanting fragrance, harbours a hidden talent as a natural preservative. This aromatic elixir boasts potent antimicrobial properties, positioning it as a formidable guardian against the microscopic threats that lurk in our food and personal care products. In the culinary realm, lavender oil emerges as an unexpected ally in the battle against spoilage. When judiciously incorporated into baked delights, Savory marinades, or zesty dressings, it acts as an invisible shield, thwarting the advances of bacteria and Mold. This natural defence mechanism not only prolongs the shelf life of our favourite foods but also reduces the risk of contamination, offering a safer gustatory experience. The skincare industry, too, has embraced lavender oil's preservative prowess. In the world of creams, lotions, and balms, this purple-hued wonder serves a dual purpose. It stands sentinel against the invasion of harmful microorganisms, extending the lifespan of these products. Simultaneously, its soothing and anti-inflammatory qualities nurture skin health, offering a holistic approach to beauty preservation. However, wielding this natural preservative requires finesse and expertise. Like any powerful tool, lavender essential oil demands respect and careful handling. Its application should be guided by the skilled hands of trained formulators who understand the delicate balance required to harness its benefits while ensuring product safety. In essence, lavender essential oil represents a harmonious fusion of nature's bounty and scientific innovation. It offers a path towards reducing our reliance on synthetic preservatives, aligning with the growing consumer demand for natural alternatives in both food and personal care products. ^54-56.

3.4 Lavender Essential Oils as Insect and Pest Control:

The fragrant essence of lavender serves as nature's own pest control, warding off a variety of common household intruders. From buzzing mosquitoes and pesky flies to fabric-munching moths and irritating fleas, lavender's aroma acts as an invisible barrier, creating a more pleasant and pest-free living environment. However, the battle against pests extends far beyond our homes, reaching into the vast expanses of agricultural lands. The relentless assault of crop-destroying pests continues to inflict significant economic wounds on the agricultural sector. This ongoing struggle has led to an increased reliance on synthetic pesticides, a trend that brings its own set of concerns. The escalating use of man-made pest control solutions has given rise to two primary issues. Firstly, there is the alarming development of pathogenic resistance. Much like antibiotic-resistant bacteria, pests are evolving to withstand these chemical onslaughts, rendering once-effective pesticides impotent. Secondly, the widespread application of these synthetic compounds raises red flags regarding human health and environmental safety. Intriguingly, research into lavender's pest-repelling properties has yielded a fascinating insight. The oil's efficacy as an insecticide appears to be tied to its chemical composition, with oxygenated compounds demonstrating more potent pest-fighting abilities compared to the hydrocarbons present in the oil. This finding opens up new avenues for developing natural, lavender-based pest control solutions that could potentially address the drawbacks of synthetic pesticides. ^57-60.

2.5 Lavender Essential Oils as Pharmaceutical and therapeutic:

Lavender essential oil, a staple in traditional medicine for centuries, has recently captured the attention of modern pharmaceutical and therapeutic researchers. This aromatic elixir is emerging as a potential powerhouse in the realm of natural remedies, with studies suggesting a wide array of therapeutic applications. At the forefront of lavender oil's potential benefits is its impact on the central nervous system. Research indicates that it may possess anxiolytic properties, helping to quell anxiety and promote more restful sleep. Beyond its calming effects, lavender oil has shown promise in addressing physical ailments, with studies exploring its anti-inflammatory, analgesic, and antimicrobial capabilities. In an era of increasing antibiotic resistance, lavender oil presents an intriguing alternative. ^61-64 Its antibacterial, antifungal, and antiviral properties have piqued the interest of researchers, who are investigating its efficacy against a spectrum of pathogens. This natural approach could potentially offer a new line of defense in our ongoing battle against harmful microorganisms. However, the therapeutic landscape of lavender oil is still being charted. While early results are promising, more comprehensive research is needed to fully unlock and understand its potential. As with any natural remedy, caution is paramount, and consultation with healthcare professionals is advised before incorporating lavender oil into any therapeutic regimen. The exploration of lavender oil's therapeutic potential comes at a critical juncture in medical research. The rising tide of inflammatory diseases, coupled with the growing specter of antibiotic resistance and the known side effects of traditional anti-inflammatory medications, has sparked a quest for alternative treatments. Traditional anti-inflammatory drugs, while effective, come with their own risks, including increased cancer susceptibility and heightened vulnerability to infections. At its core, inflammation is a intricate biological process, a complex interplay of cells and molecules. This includes cytokines, transcription factors, catabolic mediators, and pro-inflammatory genes, all working in concert to orchestrate the body's inflammatory response. Understanding and potentially modulating this process through natural means, such as lavender oil, represents an exciting frontier in medical research. ^65-68.

4. Future Potential of Lavender Essential Oil:

Lavender essential oil (LEO) exhibits significant future potential across various industries due to its diverse therapeutic properties. Research indicates that LEO possesses strong anti-inflammatory, antioxidant, and antimicrobial effects, making it a valuable candidate for pharmaceutical applications, particularly in pain management and infection control ^69,71. The incorporation of LEO in transdermal drug delivery systems has shown enhanced drug release and permeation, suggesting improved therapeutic efficacy ⁶⁹. Furthermore, encapsulation techniques can enhance LEO's stability and bioactivity, broadening its applicability in food, cosmetics, and agriculture ⁷⁰. Studies also highlight LEO's effectiveness against pathogens like Escherichia coli, indicating its potential as a natural antimicrobial agent in regions lacking access to conventional treatments ⁷². Overall, the promising bioactivity and versatility of LEO position it as a key player in the development of sustainable and effective alternatives to synthetic compounds in various sectors ^70,74.

4.1 Enhanced Therapeutic Applications:

Research into the specific mechanisms through which lavender oil exerts its effects could lead to new applications in managing mental health conditions, chronic pain, and other health issues. Advances in clinical trials and studies may reveal more precise benefits and optimal uses.

4.2 Innovations in Delivery Systems: Developments in delivery mechanisms, such as transdermal patches or novel formulations, could improve the efficacy and convenience of lavender oil applications.

4.3 Sustainable Cultivation: As demand for essential oils grows, there is increasing interest in sustainable and ethical farming practices. Innovations in cultivation and extraction methods could minimize environmental impact and ensure the quality of the oil.

5. CONCLUSION:

Lavender essential oil, with its complex chemical profile dominated by linalool and linalyl acetate, demonstrates significant versatility across multiple industries. This review has highlighted its diverse applications in cosmetics, aromatherapy, natural preservation, pest control, and pharmaceuticals. Various extraction methods, from traditional steam distillation to innovative techniques like supercritical fluid extraction, offer opportunities for optimizing yield and quality. The growing global market for lavender oil underscores its economic importance. Future research should focus on sustainable cultivation, novel delivery systems, and rigorous clinical trials to fully realize its therapeutic potential. As consumer preference for natural products increases, lavender oil is poised to play a crucial role in developing sustainable alternatives to synthetic compounds across various sectors.

6. REFERENCE:

1. Detar E., Nemeth E.Z., Gosztola B., Demjan I., Pluhar Z. Effects of variety and growth year on the essential oil properties of lavender and lavandin Biochem. Syst. Ecol. 2020; 90: 104020.

2. Shanaida M., Hudz N., Białoń M., Kryvtsowa M., Svydenko L., Filipska A., Wieczorek P.P. Chromatographic profiles and antimicrobial activity of the essential oils obtained from some species and cultivars of the Mentheae tribe (Lamiaceae) Saudi. J. Biol. Sci. 2021.

3. Luxita Sharma, Meghna Chandra and Puneeta Ajmera. Health benefits of lavender (Lavandula angustifolia). International Journal of Physiology, Nutrition and Physical Education. 2019; 4(1): 1274-1277.

4. Nelson RRS. In vitro activities of five plantessential oils against methacilh-resistant Staphylococcus aureus and vancomycin-resistant Enferococcus faecium. J Antimicrob Chemother. 1997; 40: 305-306.

5. Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Fragr J. 1998; 13: 98-104.

6. Hammer K, Carson C, Riley T. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol. 1999; 86: 985-990.

7. Rajeswara Rao B, Kaul P, Bhattacharya A, Rajput D. Comparative Chemical Composition of Steam- Distilled and Water-Soluble Essential Oils of South American Marigold (Tagetes minuta L.). J Essent Oil Res. 2006; (18): 622-626.

8. Edris AE. Identification and absolute quantification of the major water-soluble aroma components isolated from the hydrosols of some aromatic plants. J Essent Oil Bear Plants. 2009; 12(2): 155-161.

9. Prusinowska, R. and Smigielski, K. Composition, biological properties and therapeutic effects of lavender (Lavandula angustifolia L). A review. Herba Polonica. 2014; 60(2): 56-66.

10. Deren, Tahmas, Kahyaoglu., Mürüvvet, Begüm, Özen., Hilal, Yıldız. Chemical composition of essential oil extracted from lavender growing in Kastamonu, Türkiye. Turkish Journal of Forestry. 2023. doi: 10.18182/tjf.1382213

11. Fatemeh, Beheshti., Moslem, Abdipour., Mehdi, Hosseinifarahi., Abdolsamad, Kelidari. Characterization of Lavender Essential Oil and Its Chemical Composition Intercropped with Marigold as Affected by Polyamines and Arbuscular Mycorrhizal Fungi Inoculation. Communications in Soil Science and Plant Analysis. 2023 doi: 10.1080/00103624.2023.2268657

12. Ekaterina, Kozuharova., Vasil, Simeonov., Daniela, I., Batovska., Hristo, Ivanov, Valchev., Niko, Benbassat. Chemical composition and comparative analysis of lavender essential oil samples from Bulgaria in relation to the pharmacological effects. Фармация. 2023. doi: 10.3897/pharmacia.70.e104404

13. Velislava, Todorova., Kalin, Ivanov., Yoana, P., Georgieva., Diana, Karcheva-Bahchevanska., Stanislava, Ivanova. Comparison between the Chemical Composition of Essential Oil from Commercial Products and Biocultivated Lavandula angustifolia Mill. International Journal of Analytical Chemistry. 2023. doi: 10.1155/2023/1997157

14. Lavender essential oil: Nutritional, compositional, and therapeutic insights. doi: 10.1016/b978-0-323-91740-7.00009-8

15. Lu, Hui., Li, He., Lu, Huan., Li, XiaoLan., Zhou, AiGuo. Chemical composition of lavender essential oil and its antioxidant activity and inhibition against rhinitis-related bacteria. African Journal of Microbiology Research. 2010. doi: 10.5897/AJMR.9000163

16. Biljana, Kiprovski., Tijana, Zeremski., Anita, Varga., Ivana, Cabarkapa., Jelena, Filipović., Biljana, Lončar., Milica, Aćimović. Essential Oil Quality of Lavender Grown Outside Its Native Distribution Range: A Study from Serbia. Horticulturae. 2023. doi: 10.3390/horticulturae9070816

17. Daniela, Nedeltcheva-Antonova., Kamelia, Gechovska., Stanislav, Bozhanov., Liudmil, Antonov. Exploring the Chemical Composition of Bulgarian Lavender Absolute (Lavandula Angustifolia Mill.) by GC/MS and GC-FID. Plants. 2022. doi: 10.3390/plants11223150

18. Zuobing, Xiao., Li, Qi., Yunwei, Niu., Xuan, Zhou., Junhua, Liu., Yingbo, Xu., Zhiqiang, Xu. Odor-active compounds of different lavender essential oils and their correlation with sensory attributes. Industrial Crops and Products. 2017. doi: 10.1016/J.INDCROP.2017.07.040

19. Garikapati, D., Kiran, Babu., Aarti, Sharma., Bikram, Singh. Volatile composition of Lavandula angustifolia produced by different extraction techniques. Journal of Essential Oil Research. 2016. doi: 10.1080/10412905.2016.1162210.

20. Cavanagh, H. M. A., and Wilkinson, J. M. Biological activities of lavender essential oil. Phytotherapy Research. 2002; 16(4): 301-308. doi:10.1002/ptr.1103

21. Prashar, A., Locke, I. C., and Evans, C. S. Cytotoxicity of lavender oil and its major components to human skin cells. Cell Proliferation. 2004; 37(3): 221-229. doi:10.1111/j.1365-2184.2004.00310.

22. Woronuk, G., Demissie, Z., Rheault, M., and Mahmoud, S. Biosynthesis and therapeutic properties of Lavandula essential oil constituents. Planta Medica. 2011; 77(1): 7-15.

23. Wainer, J.; Thomas, A.; Chimhau, T.; Harding, K.G. Extraction of Essential Oils from Lavandula × intermedia ‘Margaret Roberts’ Using Steam Distillation, Hydrodistillation, and Cellulose-Assisted Hydrodistillation: Experimentation and Cost Analysis. Plants. 2022; 11: 3479

24. Filly, A.; Fabiano-Tixier, A.S.; Louis, C.; Fernandez, X.; Chemat, F. Water as a green solvent combined with different techniques for extraction of essential oil from lavender flowers. Comptes Rendus Chim. 2016; 19: 707–717.

25. Babu, Garikapati and Singh, Bikram. Characteristics Variation of Lavender Oil Produced by Different Hydrodistillation Techniques. Comprehensive Bioactive Natural Products-Quality Control and Standardization. 2012

26. Danh, L.T., Han, L.N., Triet, N.D.A. Comparison of Chemical Composition, Antioxidant and Antimicrobial Activity of Lavender (Lavandula angustifolia L.) Essential Oils Extracted by Supercritical CO2, Hexane and Hydrodistillation. Food Bioprocess Technol. 2013; 6: 3481–3489

27. Avşar G., Yüksel D., Emen F.M., Demirdöğen R.E., Yeşilkaynak T., Kahriman L. Supercritical carbon dioxide extraction of Lavandula officinalis (lavender) and Hypericum perforatum (centaury) plants grown in mersin region: Investigation of antioxidant and antibacterial activities of extracts and usage as cosmetic preservatives in creams. J. Turkish Chem. Soc. 2018; 5: 1215–1220.

28. G. I. Marovska, I. N. Vasileva, A. Aggelidou , N. S. Yantcheva , A. M. Slavov, Physico-chemical characteristics of polysaccharides isolated from lavender byproduct. Bulgarian Chemical Communications. 2022; 54(1): 81 - 86.

29. Ghoreishi, Seyyed and Kamali, Hossein. Supercritical Carbon Dioxide Extraction of Essential Oil From Iranian Lavender Flower. 2009

30. Rozzi, N.L., W. Phippen, J.E. Simon and R.K. Singh. Supercritical fluid extraction of essential oil components from lemon-scented botanicals. Lebensm.- Wiss. U. Technol. 2002; 35: 319-324.

31. Shang, A., Gan, R. Y., Zhang, J. R., Xu, X. Y., Luo, M., Liu, H. Y., and Li, H. B. Optimization and Characterization of Microwave-Assisted Hydro-Distillation Extraction of Essential Oils from Cinnamomum camphora Leaf and Recovery of Polyphenols from Extract Fluid. Molecules (Basel, Switzerland). 2020; 25(14): 3213.

32. Li, J. H., Li, W., Luo, S., Ma, C. H., and Liu, S. X. Alternate Ultrasound/Microwave Digestion for Deep Eutectic Hydro-distillation Extraction of Essential Oil and Polysaccharide from Schisandra chinensis (Turcz.) Baill. Molecules (Basel, Switzerland). 2019; 24(7): 1288.

33. Moradi, S., Fazlali, A., and Hamedi, H. Microwave-assisted hydro-distillation of essential oil from rosemary: Comparison with traditional distillation. Avicenna journal of medical biotechnology. 2018; 10(1): 22.

34. Vian MA, Fernandez X, Visinoni F, Chemat F. Microwave hydrodiffusion and gravity, a new technique for extraction of essential oils. Journal of Chromatography A. 2008; 1190 (1-2): 14-7.

35. López-Hortas L, Gannon L, Moreira R, Chenlo F, Domínguez H, Torres MD. Microwave hydro diffusion and gravity (MHG) processing of Laminaria ochroleuca brown seaweed. Journal of Cleaner Production. 2018; 197: 1108-16.

36. Liu, B., Fu, J., Zhu, Y., and Chen, P. Optimization of Microwave-assisted Extraction of Essential Oil from Lavender Using Response Surface Methodology. Journal of oleo Science. 2018; 67(10): 1327–1337.

37. Miladi, M.; Martins, A.A.; Mata, T.M.; Vegara, M.; Pérez-Infantes, M.; Remmani, R.; Ruiz-Canales, A.; Núñez-Gomez, D. Optimization of Ultrasound-Assisted Extraction of Spent Coffee Grounds Oil Using Response Surface Methodology. Processes. 2021; 9: 2085

38. Turrini F, Beruto M, Mela L, Curir P, Triglia G, Boggia R, Zunin P, Monroy F. Ultrasound-Assisted Extraction of Lavender (Lavandula angustifolia Miller, Cultivar Rosa) Solid By-Products Remaining after the Distillation of the Essential Oil. Applied Sciences. 2021; 11(12): 5495.

39. Richa, Rishi and Kumar, Rohitashw and Shukla, Rakesh and Khan, Kalay. Ultrasound assisted essential oil extraction Technology. New Boon in Food Industry. 2020; 22: 81-85.

40. Fathy Sayed Morsy, N. Essential Oil as Green Preservative Obtained by Ecofriendly Extraction Techniques. Intechopen. 2022. Doi: 10.5772/intechopen.103035

41. Rashed, Marwan and Tong, Qunyi and Rotail, Ashraf and Al-Farga, Ammar and Aboshora, Waleed and Al-Hajj, Dr-Nabil. Extraction of essential oil from Lavandula angustifolia flowers preceded by enzymatic pre-treatment and investigate its activity against free radicals. International Journal of Research in Agricultural Sciences. 2017; 4: 106-110.

42. Sajja, Hari and Srinivas, N and Raghavarao, Ksms and Karanth, Naikankatte. Reverse Micellar Extraction for Downstream Processing of Proteins/Enzymes. Advances in Biochemical Engineering/ Biotechnology. 2002; 75: 119-83. 10.1007/3-540-44604-4_

43. Adrian and Santacroce, Luigi and Jacob, Romeo and Mare, Anca and Man, Lidia. Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study. Pathogens. 2019; 8: 15. 10.3390/pathogens8010015.

44. Ghoreishi, S. and Kamali, H. Supercritical Carbon Dioxide Extraction of Essential Oil from Iranian Lavender Flower. 2009.

45. Rashed, M., Tong, Q., Rotail, A., Al-Farga, A., Aboshora, W., and Al-Hajj, N. Extraction of essential oil from Lavandula angustifolia flowers preceded by enzymatic pre-treatment and investigate its activity against free radicals. International Journal of Research in Agricultural Sciences. 2017; 4: 106-110.

46. Sajja, H., Srinivas, N., Raghavarao, K.S.M.S., and Karanth, N. Reverse Micellar Extraction for Downstream Processing of Proteins/Enzymes. Advances in Biochemical Engineering/ Biotechnology. 2002; 75: 119-83.

47. Cavanagh HM, Wilkinson JM. Biological effects of lavender essential oil. Phytotherapy Research. 2002; 16: 301-308.

48. Carvalho IT, Estevinho BN, Santos L. Application of microencapsulated essential oils in cosmetic and personal healthcare products – A review. International Journal of Cosmetic Science. 2015; 38: 109-119.

49. Herrera AG. Microbiological analysis of cosmetics. Methods in Molecular Biology. 2004; 268: 293–295.

50. Amaral LF, Camilo NS, Pereda MDCV, Levy CE, Moriel P, Mazzola PG. Evaluation of antimicrobial effectiveness of C-8 xylitol monoester as an alternative preservative for cosmetic products. International Journal of Cosmetic Science. 2011; 33: 391–397.

51. Anderson F. Final amended report on the safety assessment of methylparaben, ethylparaben, propylparaben, isopropylparaben, butylparaben, isobutylparaben, and benzylparaben as used in cosmetic products. International Journal of Toxicology. 2008; 27: 1-82.

52. Wells R, Truong F, Adal AM, Sarker LS, Mahmoud SS. Lavandula Essential Oils: A Current Review of Applications in Medicinal, Food, and Cosmetic Industries of Lavender. Natural Product Communications. 2018; 13(10).

53. Steflitsch W, Steflitsch M. Clinical aromatherapy. Journal of Men’s Health. 2008; 5: 74–85.

54. Zhang Y, Wu Y, Chen T, Yao L, Liu J, Pan X, Hu Y, Zhao A, Xie G, Jia W. Assessing the metabolic effects of aromatherapy in human volunteers. Evidence-Based Complementary and Alternative Medicine. 2013; 1-9.

55. Barcan R. Aromatherapy oils: commodities, materials, essences. Cultural. Studies Review. 2014: 20: 141-171.

56. Buchbauer G, Jirovetz L, Jäger W, Dietrich H, Plank C. Aromatherapy: Evidence for sedative effects of the essential oil of lavender after inhalation. Zeitschrift fϋr Naturforschu C. 1991; 46: 1067–1072.

57. Fismer KL, Pilkington K. Lavender and sleep: A systematic review of the evidence. European Journal of Integrative Medicine. 2012; 4: E436–E447.

58. Djenane D, Yangüela J, Derriche F, Bouarab L, Roncales P. Olive tree leaf extract; in vitro tests on Staphylococcus aureus, Salmonella enterditis and Pseudomonas aeruginosa; application in turkey meat. Phytothérapie. 2012; 10: 10–18.

59. Ait-Ouazzou A, Cherrat L, Espina L, Lorán S, Rota C, Pagán R. The antimicrobial activity of hydrophobic essential oil constituents acting alone or in combined processes of food preservation. Innovative Food Science and Emerging Technologies. 2011; 12: 320–329.

60. Yohalem D, Passey T. Amendment of soils with fresh and post-extraction lavender (lavandula angustifolia) and lavandin (lavandula×intermedia) reduce inoculum of verticillium dahliae and inhibit wilt in strawberry. Applied Soil Ecology. 2011; 49: 187–196.

61. Kadoglidou K, Lagopodi A, Karamanoli K, Vokou D, Bardas GA, Menexes G, Constantinidou HIA. Inhibitory and stimulatory effects of essential oils and individual monoterpenoids on growth and sporulation of four soil-borne fungal isolates of Aspergillus terreus, Fusarium oxysporum, Penicillium expansum, and Verticillium dahliae. European Journal of Plant Pathology. 2011; 130: 297–309.

62. Simunková, K., Hysek, S., Reinprecht, L. Et al. Lavender oil as eco-friendly alternative to protect wood against termites without negative effect on wood properties. Sci Rep. 2022; 12: 1909.

63. Papachristos DP, Stamopoulos DC. Selection of Acanthoscelides obtectus (Say) for resistance to lavender essential oil vapour. Journal of Stored Products Research. 2003; 39: 433–441.

64. Papachristos DP, Karamanoli KI, Stamopoulos DC, Menkissoglu-Spiroudi U. The relationship between the chemical composition of three essential oils and their insecticidal activity against Acanthoscelides obtectus (Say). Pest Management Science. 2004; 60: 514-520.

65. Bohinc T, Vayias B, Bartol T, Trdan S. Assessment of insecticidal efficacy of diatomaceous earth and powders of common lavender and field horsetail against bean weevil adults. Neotropical Entomology. 2013; 42: 642–648

66. Zuzarte M, Gonçalves MJ, Cavaleiro C, Cruz MT, Benzarti A, Marongiu B, Maxia A, Piras A, Salgueiro L. Antifungal and anti-inflammatory potential of Lavandula stoechas and Thymus herba-barona essential oils. Industrial Crops and Products. 2013; 44: 97–103.

67. Williamson B, Tudzynski B, Tudzynski P, Van Kan JA. Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology. 2007; 8: 561–580.

68. Soylu EM, Soylu S, Kurt S. Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent phytophthora infestans. Mycopathologia. 2006; 161: 119–128.

69. Baker J, Brown K, Rajendiran E, Yip A, decoffe D, Dai C, Molcan E, Chittick SA, Ghosh S, Mahmoud S, Gibson DL. Medicinal lavender modulates the enteric microbiota to protect against citrobacter rodentium-induced colitis. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2012; 303: G825–836.

70. Darshan S, Doreswamy R. Patented antiinflammatory plant drug development from traditional medicine. Phytotherapy Research. 2004; 18: 343–57

71. Karolina, Zyburtowicz., Paulina, Bednarczyk., Anna, Nowak., Anna, Muzykiewicz-Szymańska., Łukasz, Kucharski., Aneta, Wesołowska., Paula, Ossowicz‐Rupniewska. Medicinal Anti-Inflammatory Patch Loaded with Lavender Essential Oil. International Journal of Molecular Sciences. 2024. doi: 10.3390/ijms25116171

72. S, M, Hedayati, Mohammad, Tarahi., Aida, Iraji., Mohammad, Hashem, Hashempur. Recent developments in the encapsulation of lavender essential oil. Advances in Colloid and Interface Science. 2024. doi: 10.1016/j.cis.2024.103229

73. Anelia, Mladenova. Lavender essential oil - properties and uses. 2023. doi: 10.61308/nngn3937

74. Allyson, Muller, Shreya, Dhara., Paige, Herrin., Paul, Muller. The Effect of Lavender Essential Oil on Escherichia coli Growth and its Potential Applications. Journal of Student Research. 2023 doi: 10.47611/jsrhs.v12i3.4744

Received on 19.08.2024 Revised on 26.09.2024

Accepted on 28.10.2024 Published on 25.11.2024

Available online from December 27, 2024

Asian J. Research Chem. 2024; 17(6):377-386.

DOI: 10.52711/0974-4150.2024.00063

Sr. No	Extraction Method	Typical Yield (% of dry weight)	Characteristics	Advantages	Disadvantages
1	Steam Distillation	1-3%	Uses steam to separate oil	Low investment cost, simple process	Energy-intensive, may alter heat-sensitive compounds
2	Hydro Distillation	0.2-1.5%	Flowers soaked in water before heating	Better quality oil than steam distillation	Lower yield than steam distillation, time-consuming
3	Solvent Extraction	3.0-5.0 %	Uses organic solvents like hexane	Higher yields possible	Risk of solvent residues, environmental concerns
4	Supercritical Fluid Extraction	0.3-4.5%	Uses supercritical CO₂	Environmentally friendly, high-quality oil	High initial investment, complex process control
5	Microwave-Assisted Extraction	0.6-2.5%	Uses microwave energy	Rapid extraction, preserves volatile compounds	Requires specialized equipment
6	Ultrasound-Assisted Extraction	0.5-3.5%	Uses high-frequency sound waves	Efficient, may yield higher chemical complexity	Potential degradation of some compounds
7	Enzymatic Extraction	0.5-3.5%	Uses enzymes to break down cell walls	Gentle method, preserves heat-sensitive compounds	Time-consuming, enzyme cost
8	Micellar extraction	1.5-3.5 %	Using surfactant	Selective Extraction and High yield	Complexity and high cost

1. INTRODUCTION: