ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): G.V.H. Raju, S. Ganapathy, D.G. Sankar, P.Y. Naidu

Email(s): gadepalli_raj@yahoo.com

DOI: Not Available

Address: G.V.H. Raju*, S. Ganapathy, D.G. Sankar and P.Y. Naidu
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
A simple Reverse Phase liquid chromatographic(HPLC) method for the determination of Levetiracetam in human plasma has been developed. Separation was achieved on a Prontosil C8 (150 x 4.6 mm, 5 µm) column with mobile phase composition of [pH 3.8 buffer: acetonitrile] (Solution A): HPLC grade water (Solution B) in the ratio 60:40 v/v with ultra violet detection at 210nm. Lower limit of quantitation was 200ng/ml.Maximum between-run precision was 5.91%. Mean extraction recovery was found to be 100.02 to 103.06%. Stability study showed that after three freeze-thaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 48h and at -20° for 2months. Before injecting onto HPLC system, the processed samples were stable for at least 6h.The method was used to perform bioequivalence study in human.


Cite this article:
G.V.H. Raju, S. Ganapathy, D.G. Sankar, P.Y. Naidu. Development and Validation of an HPLC Method for Analysis of Levetiracetam in Human Plasma. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 776-780.

Cite(Electronic):
G.V.H. Raju, S. Ganapathy, D.G. Sankar, P.Y. Naidu. Development and Validation of an HPLC Method for Analysis of Levetiracetam in Human Plasma. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 776-780.   Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2010-3-3-67


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Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

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