Author(s):
Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.
Email(s):
shinde.ganesh904@gmail.com
DOI:
10.52711/0974-4150.2024.00019 
Address:
Shinde G. S.1*, Jadhav R. S. 1, Kote R. B.1, Kadam A. J.2, Bankar A. A.2
1Department of Pharmaceutics, Institute of Pharmacy Loni. Tal-Rahata, District - Ahmednagar. (M.S.) 413736.
2Department of Pharmaceutical Chemistry, Institute of Pharmacy Loni, Taluka-Rahata District-Ahmednagar. (M.S.) 413736.
*Corresponding Author
Published In:
Volume - 17,
Issue - 2,
Year - 2024
ABSTRACT:
Purpose: To develop a simple, sensitive, accurate, precise and rapid UV spectrophotometric method for the determination of metformin in human urine. Methods: Extraction of metformin from urine samples was done by Dilute and shoot method in which urine was diluted up to 1:10 ratio to avoid interference of matrix. For estimation of Metformin hydrochloride, the solvent system employed was water. The metformin hydrochloride was scanned in range of 200nm to 400 nm and wavelength of detection (?det) was found to be 234 nm. Result: The calibration curve was linear in the range of 1-17 µg/ml. the recovery and assay studies of metformin hydrochloride were within 93-94.85% indicating that the proposed method can be estimation of metformin hydrochloride
Cite this article:
Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.. Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample. Asian Journal of Research in Chemistry. 2024; 17(2):93-6. doi: 10.52711/0974-4150.2024.00019
Cite(Electronic):
Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.. Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample. Asian Journal of Research in Chemistry. 2024; 17(2):93-6. doi: 10.52711/0974-4150.2024.00019 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2024-17-2-7
REFERENCE:
1. Ganesh Shinde, Godage R. K, R. S. Jadhav, Barhate Manoj, Bhagwat Aniket. A Review on Advances in UV Spectroscopy. Research J. Science and Tech. 2020; 12(1): 47-51. doi: 10.5958/2349-2988.2020.00005.4
2. Grzybowska M, Bober J, Olszewska M. Metformin - mechanisms of action and use for the treatment of type 2 diabetes mellitus. Postepy Hig Med Dosw online. 2011; 6(65): 277-85.
3. Kar M, Choudhury PK. HPLC method for estimation of metformin hydrochloride in formulated microspheres and tablet dosage form. Indian J Pharm Sci. 2009; 71(3): 318–20.
4. Charles BG, Jacobsen NW, Ravenscroft PJ. Rapid liquid-chromatographic determination of metformin in plasma and urine. Clin Chem 1981; 27: 434-436.
5. Matin SB, Karam JH, Forshan PH. Simple electron capture gas chromatographic method for the determination of oral hypoglycemic biguanidis in biological fluids. Anal Chem. 1975; 47: 545-548.
6. International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use. Validation of Analytical Procedures; Text and Methodology ICH Q2 [R1]; 2005.
7. Saad Aantakli, Leon Nejem, Monzer ALraii. New Spectrophotometric Gliclazide Determination in Tablets Formulations by Charge Transfer Complexation. Research Journal of Pharmacy and Technology. 2023; 16(4): 1714-0. doi: 10.52711/0974-360X.2023.00282