Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez
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Imene Guediri1,4, Chérifa Boubekri2,4*, Ouanissa Smara1,5 and Touhami Lanez3,4
1University Kasdi Merbah, Faculty of Material Science, Department of Chemistry, Ghardaia BP Road 511. 30,000, Ouargla, Algeria
2*University Mohamed Khider, Faculty of Exact sciences, Natural and Lile Sciences, Department of Material sciences, BP145 RP Biskra 07000 Algeria.
3University of Echahid Hamma Lakhdar, Faculty of Exact sciences, PO Box 789, 39000, El Oued, Algeria
4University of El Oued, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria
5Laboratory Valorisation et Promotion des Ressources Sahariennes (VPRS), 30000, Ouargla, Algeria
Volume - 14,
Issue - 1,
Year - 2021
In the present research, we studied the quantification of the total phenolics, total flavonoids, total flavonols, and the evaluation of the antioxidant activity of the fruits of Solanum nigrum L. by chemical and electrochemical methods. The chloroform, ethyl acetate, and n-butanol crude extracts are prepared from the hydroethanolic extract of the fruits were tested. The results showed that ethyl acetate extract showed the highest phenolic content (87.68mg GAE/g), chloroform extract showed the highest flavonoid content (24.23mg QE/g) and highest flavonol content (71.60mg RE/g). The evaluation of antioxidant activity by chemical methods revealed that the highest DPPH radical scavenging activity was shown by ethyl acetate extract (0.123mg/mL) while the highest ferric reducing capacity was shown by butanol extract (610.46±0.0015mg FeSO4/g). The electrochemical study of the three extracts by cyclic voltammetry method indicates that the highest antioxidant activity was shown by ethyl acetate extract (17.75±0.238mg/g) equivalent ascorbic acid. The superoxide scavenging assay of chloroform extract showed the highest value (0.8 mg/mL).
Cite this article:
Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez. Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (black nightshade) growing in the south of Algeria. Asian J. Research Chem. 2021; 14(1):47-55. doi: 10.5958/0974-4150.2021.00008.0
Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez. Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (black nightshade) growing in the south of Algeria. Asian J. Research Chem. 2021; 14(1):47-55. doi: 10.5958/0974-4150.2021.00008.0 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2021-14-1-8
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