0974-4150 (Online)
0974-4169 (Print)

Author(s): Amar Djemoui, Djamila Djemoui, Lahcene Souli, Ahmed Souadia, Messaoud Gouamid

Email(s): ,

DOI: 10.52711/0974-4150.2021.00032   

Address: Amar Djemoui1*, Djamila Djemoui2, Lahcene Souli1, Ahmed Souadia1, Messaoud Gouamid3
1Laboratory of Organic Chemistry and Natural Substance, Department of Chemistry, Faculty of Exact Sciences and informatics, ZIANE Achour University of Djelfa (Algeria).
2Department of Chemistry, Faculty of Exact Sciences, Echahid Hamma Lakhdar University of El Oued, Algeria.
3Department of Chemistry, Faculty of Mathematics and Matter Sciences, Kasdi Merbah, University of Ouargla, Algeria.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021

Moringa oleifera Flowers extracts grown in Algerian Sahara were evaluated antidiabetic and antioxidant activity by means using divers established in vitro systems, such as a-Amylase inhibitory assay, DPPH radical scavenging assay, Phosphomolybdenum assay (PM) and Ferric reducing power assay (FRAP). Moreover, the total content of phenols, flavonoids and tannins from (MFCE) and various fractions was measured using colorimetric methods. Results demonstrated that TPC varied between 95,50 ? 0,42 and 10, 49 ? 0,053 mg GAE/g WE, while TFC was between 17,00 ? 0, 011 and 2,47 ? 0,014 mg GAE/g WE, In this study TTC ranged between 2,96? 0,016 and 1,30? 0, 014 mg GAE/g WE. All capacities of DPPH radical scavenging, Phosphomolybdenum (PM) and Ferric reducing power (FRAP) were found best in (MFEF) (IC50= 0,159± 0,004mg/ml, AEAC = 42.37 ± 0.28 mM and AEAC =104.05±0.41 mM respectively). Add to this (MFEF) showed the highest a-amylase inhibitory activity (I= 38,92 %).

Cite this article:
Amar Djemoui, Djamila Djemoui, Lahcene Souli, Ahmed Souadia, Messaoud Gouamid. The Antidiabetic, Antioxidant properties in vitro of Moringa oleifera Flowers extracts grown in Sahara of Algeria. Asian Journal of Research in Chemistry.2021; 14(3):173-8. doi: 10.52711/0974-4150.2021.00032

Amar Djemoui, Djamila Djemoui, Lahcene Souli, Ahmed Souadia, Messaoud Gouamid. The Antidiabetic, Antioxidant properties in vitro of Moringa oleifera Flowers extracts grown in Sahara of Algeria. Asian Journal of Research in Chemistry.2021; 14(3):173-8. doi: 10.52711/0974-4150.2021.00032   Available on:

1.    Olson, M.E., Combining data from DNA sequences and morphology for a phylogeny of Moringaceae (Brassicales). Systematic Botany. 2002. 27(1): p. 55-73.
2.    Anwar, F., and al., Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2007. 21(1): p. 17-25.
3.    Fuglie, L.J., The miracle tree: Moringa oleifera, natural nutrition for the tropics. 1999.
4.    Sutalangka, C., and al., Moringa oleifera mitigates memory impairment and neurodegeneration in animal model of age-related dementia. Oxidative medicine and cellular longevity, 2013. 2013.
5.    Arulmozhi, K. and L. Wilson, In vitro antioxidant properties of Moringa oleifera and Tagetes erecta flower extracts. International Journal of Current Microbiology and Applied Sciences. 2015. 4(10): p. 733-740.
6.    Herborne, J., Phytochemical methods. A guide to modern techniques of plant analysis, 1973: p. 5-11.
7.    Savithramma, N., M.L. Rao, and D. Suhrulatha, Screening of medicinal plants for secondary metabolites. Middle-East Journal of Scientific Research. 2011. 8(3): p. 579-584.
8.    Yadav, R. and M. Agarwala, Phytochemical analysis of some medicinal plants. Journal of phytology. 2011.
9.    Al-Owaisi, M., N. Al-Hadiwi, and S.A. Khan, GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pacific Journal of Tropical Biomedicine. 2014. 4(12): p. 964-970.
10.    Wang, H., and al., In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chemistry. 2008. 106(3): p. 888-895.
11.    Preeti Tiwari. Phenolics and Flavonoids and Antioxidant Potential of Balarishta Prepared by Traditional and Modern Methods. Asian J. Pharm. Ana. 4(1): Jan.-Mar. 2014; Page 05-10.
12.    Djemoui, D, Saidi, M., Rahmani, Z. and Djemoui A., Influence of phenolic compounds on antioxidant capacity of leaves extracts of Moringa oleifera from tamanrasset region. Journal of Fundamental and Applied Sciences. 2019, 11(1): p.280-293;
13.    Rajurkar RM, Jain RG, Bedmohta PA, Khadbadi SS. Antioxidant Activity of Phenolic Extract from Ginger (Zingiber officinale Roscoe) Rhizome. Asian J. Research Chem. 2(3): July-Sept. 2009 page 260-261
14.    Prieto, P., M. Pineda, and M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 1999. 269(2): p. 337-341.
15.    Jiby Elias, Rajesh MG, Anish NP, Deepa P, Jayan N. In Vitro Antioxidant Activity of the Methanolic Extract of Simaruba glauca DC. Asian J. Research Chem. 3(2): April- June 2010; Page 312-315.
16.    Ademiluyi, A.O., et al., Effect of fermented soybean condiment supplemented diet on α-amylase and α-glucosidase activities in Streptozotocin-induced diabetic rats. Journal of Functional Foods, 2014. 9: p. 1-9.
17.    Vermerris, W. and R. Nicholson, Phenolic compound biochemistry2007: Springer Science & Business Media.
18.    Quideau, S., Flavonoids. Chemistry, Biochemistry and Applications. Edited by Øyvind M. Andersen and Kenneth R. Markham. Angewandte Chemie International Edition. 2006. 45(41): p. 6786-6787.
19.    De Bruyne, T., et al., Condensed vegetable tannins: biodiversity in structure and biological activities. Biochemical Systematics and Ecology. 1999. 27(4): p. 445-459.
20.    Scalbert, A., Antimicrobial properties of tannins. Phytochemistry. 1991. 30(12): p. 3875-3883.
21.    Thastrup, O., et al., Inhibition of human platelet aggregation by dihydropyrano-and dihydrofuranocoumarins, a new class of cAMP-phosphodiesterase inhibitors. Biochemical pharmacology. 1985. 34(12): p. 2137-2140.
22.    Thornes, R., Clinical and biological observations associated with coumarins. Coumarins: biology, applications and mode of action. John Wiley & Sons, Inc., New York, NY, 1997: p. 256.
23.    Smyth, T., V. Ramachandran, and W. Smyth, A study of the antimicrobial activity of selected naturally occurring and synthetic coumarins. International Journal of Antimicrobial Agents. 2009. 33(5): p. 421-426.
24.    Dai, J. and R.J. Mumper, Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010. 15(10): p. 7313-7352.
25.    M. Vijayabaskaran, G. Babu, N. Venkateswaramurthy, P. Sivakumar, P. Perumal, T. Sivakumar, R. Sambath Kumar, B. Jayakar. Antitumor Activity and Antioxidant Role of Ethanolic Extract of Pseudarthria viscida Linn against Ehrlich Ascites Carcinoma. Asian J. Research Chem. 4(1): January 2011; Page 110-113.
26.    Turro, N.J., M. Aikawa, and I.R. Gould, The laser vs. the lamp: a novel laser-induced adiabatic reaction and luminescence of benzophenones. Journal of the American Chemical Society. 1982. 104(3): p. 856-858.
27.    Kim, J., et al., Oxidative modification of cytochrome c by singlet oxygen. Free Radical Biology and Medicine. 2008. 44(9): p. 1700-1711.
28.    Shlini P., Siddalinga Murthy K.R. Extraction of Phenolics, Proteins and Antioxidant Activity from Defatted Tamarind Kernel Powder. Asian J. Research Chem. 4(6): June, 2011; Page 936-941.
29.    Perron, N.R., et al., Predicting how polyphenol antioxidants prevent DNA damage by binding to iron. Inorganic Chemistry. 2008. 47(14): p. 6153-6161
30.    Muanda, F., et al., Phytochemical composition and antioxidant capacity of three malian medicinal plant parts. Evidence-Based Complementary and Alternative Medicine. 2011. 2011.
31.    Keffous, F., et al., Determination of the antioxidant activity of Limoniastrum feei aqueous extract by chemical and electrochemical methods. Cogent Chemistry. 2016. 2(1): p. 1186141.
32.    S. Subramanian, S. Rajeswari, G. Sriram Prasath. Antidiabetic, Antilipidemic and Antioxidant Nature of Tridax procumbens Studied in Alloxan-Induced Experimental Diabetes in Rats: a Biochemical Approach. Asian J. Research Chem. 4(11): Nov., 2011; Page 1732-1738.
33.    DE Graft; Johnson, J., et al., Ferric‐reducing ability power of selected plant polyphenols and their metabolites: implications for clinical studies on the antioxidant effects of fruits and vegetable consumption. Basic and Clinical Pharmacology ans Toxicology, 2007. 100(5): p. 345-352.
34.    Sonali Munne, D.V. Parwate, V.N. Ingle, D.S. Panchbhai, V.S. Nagpurkar. Free Radical Scavenging Activity of Gamma Irradiated and Unirradiated Citrus medica Empty Juice Sacs. Asian J. Research Chem. 4(6): June, 2011; Page 957-959.
35. Ghanshyam B. Jadhav, Ravindranath B. Saudagar. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian J. Res. Pharm. Sci. 4(2): April-June 2014; Page 51-54.
36.    Benfeld, P., Amylases a and ß. Method Enzymol, 1955. 1: p. 149-58.
37.    M Sugumaran, T Vetrichelvan, S Darlin Quine. Antidiabetic potential of aqueous and alcoholic leaf extracts of Pithecellobium dulce. Asian J. Research Chem. 2(1): Jan.-March, 2009; Page 83-85.
38.    McDougall, G.J., et al., Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase. Journal of Agricultural and Food Chemistry, 2005. 53(7): p. 2760-2766.
39.    Hedqvist, H., Metabolism of soluble proteins by rumen microorganisms and the influence of condensed tannins on Nitrogen Solubility and Degradation. Vol. 501. 2004.
40.    Kandra, L., et al., Inhibitory effects of tannin on human salivary α-amylase. Biochemical and Biophysical Research Communications. 2004. 319(4): p. 1265-1271.
41.    RB Goswami, Neelima Goswami, P Khare, S Choudhary, AK Pathak. Evaluation of Anti-Diabetic Activity of Leaves and Fruits of Ficus religiosa Linn. Research J. Pharmacognosy and Phytochemistry, 2010; 2(1): 61-63.

Recomonded Articles:

Author(s): Arun Kumar, Vinita Gupta, Sanchita Singh, Y.K. Gupta

DOI: 10.5958/0974-4150.2017.00038.4         Access: Open Access Read More

Author(s): Anshul Sinha, A. K. Meena, P. Panda, Bhavana Srivastava, M. D. Gupta, M. M. Padhi

DOI:         Access: Open Access Read More

Author(s): Tauseef Shaikh, Atar Mujum , Khan Wasimuzzama, Rukhsana A Rub

DOI:         Access: Open Access Read More

Author(s): Balakrishnan N, Panda A B, Raj N R, Shrivastava A, Prathani R

DOI:         Access: Open Access Read More

Author(s): K.P. Beena, G. Sathya Pooja

DOI: 10.52711/0974-4150.2022.00030         Access: Closed Access Read More

Author(s): Trapti Rastogi, Vijay Bhutda, Komal Moon, PB Aswar, SS Khadabadi

DOI:         Access: Open Access Read More

Author(s): Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V

DOI: 10.5958/0974-4150.2019.00062.2         Access: Open Access Read More

Author(s): Gangadhara MM, Dinesha R, Satish Kumar Murari

DOI: 10.5958/0974-4150.2017.00020.7         Access: Open Access Read More

Author(s): Anitha Jebamalai Raj, Sudarsanam Dorairaj

DOI:         Access: Open Access Read More

Author(s): S Kalidas, B Kameswari, P Devi, B Madhumitha, R Meera, NJ Merlin

DOI:         Access: Open Access Read More

Author(s): Shlini P., Siddalinga Murthy K.R.

DOI:         Access: Open Access Read More

Author(s): Uday M. Satpute, Sachin H. Rohane

DOI: 10.5958/0974-4150.2021.00016.X         Access: Open Access Read More

Author(s): Vijay N. Bhosale, Gopinath S. Khansole, Jaman A. Angulwar, Sunil S. Choudhare, Ashok R. Karad, Navanand B. Wadwale

DOI: 10.5958/0974-4150.2017.00126.2         Access: Open Access Read More

Author(s): Sonu Jacob, Anil John J., Leena Thomas, Sabulal B.

DOI:         Access: Open Access Read More

Author(s): Onwudiwe, N. N., Njoku, O. U., Joshua, P. E.

DOI:         Access: Open Access Read More

Author(s): K. Radhika, B. Kumaravel, V. Thamizhiniyan, S. Subramanian

DOI:         Access: Open Access Read More

Author(s): Rakesh Saini, Saurabh Chaturvedi, M. Shahar Yar, H. P. Bhartiya, Pratibha Singh

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles

Recent Articles