ABSTRACT:
A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of fluoxetine hydrochloride in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 226nm.The developed method was validated with respect to linearity, accuracy (recovery), precision, specificity, ruggedness and robustness. Beer’s law was obeyed in the concentration range of 5-50µg/ml having line equation y =0.027x+0.030 with correlation coefficient of 0.999. The percentage recovery is 100.01 which reflect that the method is free from interference of the impurities and other additives during the estimation of drug in formulation. The proposed method can be successfully used for analysis of fluoxetine hydrochloride in marketed preparations. Results of the analysis were validated statistically and by recovery study.
Cite this article:
Zahid Zaheer, Obaid Shaikh, Sucheta Thorat, Rana Z. Ahmed. Development and Validation of UV Spectrophotometric Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 545-548.
Cite(Electronic):
Zahid Zaheer, Obaid Shaikh, Sucheta Thorat, Rana Z. Ahmed. Development and Validation of UV Spectrophotometric Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 545-548. Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2010-3-3-6