Author(s):
Mohmedyasin F. Mansuri, Shravan B. Rathod
Email(s):
shravanathorizon93@gmail.com
DOI:
10.5958/0974-4150.2019.00054.3 
Address:
Mohmedyasin F. Mansuri1, Shravan B. Rathod2*
1Department of Microbiology, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India
2Department of Chemistry, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India
*Corresponding Author
Published In:
Volume - 12,
Issue - 6,
Year - 2019
ABSTRACT:
The Fluorescence Resonance Energy Transfer (FRET) between 2,5-diphenyl-1,3-oxazole (PPO) and 7-amino-4-(trifluoromethyl) chromen-2-one (Coumarin 151) dyes was investigated. In these studies, PPO behaves as a donor and Coumarin 151 as an acceptor. All the measurements were carried out in DMSO solvent. The quantum yield of the donor was found 0.48 using tryptophan as a standard at 280 nm excitation wavelength. Energy transfer was confirmed by excitation spectra, steady-state fluorescence quenching, and Time-correlated single photon counting measurements. Measured Förster distance (R0) and FRET efficiency (E) of the system are observed 33 Å and 0.24 respectively. Binding energies of the donor, acceptor and their derivatives with two conformers of Prolyl-tRNA synthetase (PDB: 4TWA, 4YDQ) are found different in magnitudes by docking studies.
Cite this article:
Mohmedyasin F. Mansuri, Shravan B. Rathod. Förster Resonance Energy Transfer Investigation between PPO and Coumarin 151 Dyes and Docking studies with Prolyl-tRNA Synthetase Conformers. Asian J. Research Chem. 2019; 12(6):295-302. doi: 10.5958/0974-4150.2019.00054.3
Cite(Electronic):
Mohmedyasin F. Mansuri, Shravan B. Rathod. Förster Resonance Energy Transfer Investigation between PPO and Coumarin 151 Dyes and Docking studies with Prolyl-tRNA Synthetase Conformers. Asian J. Research Chem. 2019; 12(6):295-302. doi: 10.5958/0974-4150.2019.00054.3 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2019-12-6-2