0974-4150 (Online)
0974-4169 (Print)

Author(s): Naima Boukraa, Segni Ladjel, Mohamed Bilal Goudjil, Amar Eddoud, Kifah Waleed Mustafa Sanori


DOI: 10.5958/0974-4150.2020.00081.4   

Address: Naima Boukraa1,2*, Segni Ladjel1,3, Mohamed Bilal Goudjil1,3, Amar Eddoud4,5, Kifah Waleed Mustafa Sanori6
1Kasdi Merbah University, Faculty of Applied Sciences, Process Engineering Laboratory, Ouargla, Algeria.
2Kasdi Merbah University, Faculty of Natural Sciences and Life Sciences, Biological Sciences Department, Ouargla, Algeria.
3Kasdi Merbah University, Faculty of Applied Sciences, Department of Process Engineering, Ouargla 30000, Algeria.
4Kasdi Merbah University, Faculty of Natural Sciences and Life Sciences, Department of Agronomic Sciences, Ouargla, Algeria.
5Kasdi Merbah University, Faculty of Natural Sciences and Life Sciences, Saharan bio-resources laboratory, Ouargla, Algeria.
6Universiti Sains Malysia, School of Languages Literacies and Translation, Penang, Malaysia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 6,     Year - 2020

The chemical composition of the essential oils (EOs) of Artemisia herba-alba Asso, Rosmarinus officinalis L and Juniperus pheonicea L and its fumigant and repellent activities against Tribolium castaneum (Herbst) were investigated as well as their mixture. Dried leaves and flowers were subjected to hydrodistillation using a Clevenger-type apparatus and the chemical composition of the volatile oils was studied by gas chromatography-mass spectrometry (GC-MS). A total of 117 components were identified from the three EOs and their mixture. The main compounds identified for EOs of A. herba alba, R. officinalis, J. pheonicea and the mixture were Car-3-en-5-one (12.51%), Camphor (34.37%), a-Pinene (15.49%), and camphor (13.95%), respectively. it Interestingly we discovered that the EO extracted from A. herba alba from Khenchela had a new chemotype with the appearance of new compounds in the mixture of EOs. Results of fumigant toxicity showed that after 24 h exposure time, all EOs had pronounced toxicity against T. castaneum, with LC50 value of 107.199, 155.724 and 156.589µl/l air for A. herba-alba, J. pheonicea and R. officinalis, respectively, while the LC50 of the mixture was 131.570µl/l air. However, at the concentration 2µl and after 30 min exposure time, the EOs of A. herba alba and J. phoenicea showed significantly more repellent effects up to 100.00±0.00 and 90.00±10.00%, respectively, followed by the mixture and the R. officinalis EO with the percentages of repulsion equal to 76.67±11.55 and 41.67±10.41% . Data analysis by CompuSyn showed that the synergy, antagonism or additive effect of the EOs in both tests depend on the concentration of the bioinsecticides and the exposure time. The results indicated that the tested EOs and their mixture had the potential to be developed as bioinsecticides and repellent for the control of T. castaneum adults.

Cite this article:
Naima Boukraa, Segni Ladjel, Mohamed Bilal Goudjil, Amar Eddoud, Kifah Waleed Mustafa Sanori. Chemical compositions, Fumigant and Repellent Activities, of Essential oils from three Indigenous medicinal plants and their mixture, against stored grain pest, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Asian J. Research Chem. 2020; 13(6):455-464. doi: 10.5958/0974-4150.2020.00081.4

Naima Boukraa, Segni Ladjel, Mohamed Bilal Goudjil, Amar Eddoud, Kifah Waleed Mustafa Sanori. Chemical compositions, Fumigant and Repellent Activities, of Essential oils from three Indigenous medicinal plants and their mixture, against stored grain pest, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Asian J. Research Chem. 2020; 13(6):455-464. doi: 10.5958/0974-4150.2020.00081.4   Available on:

1.    Jaya, Singh P, Prakash B, Dubey NK. Insecticidal activity of Ageratum conyzoides L., Coleus aromaticus Benth. and Hyptis suaveolens (L.) Poit essential oils as fumigant against storage grain insect Tribolium castaneum Herbst. J Food Sci Technol. 2012; 51(9): 2210‐2215.
2.    Brari J, Thakur DR. Fumigation and repellent potential of four essential oils against major coleopteran pests of stored grain. International Journal of Advance Agricultural Research. 2017; 5: 40-51.
3.    Chaubey MK. Acute, Lethal and Synergistic Effects of Some Terpenes Against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Ecologia Balkanica. 2012; 4(1): 53-62.
4.    Sener O, Arslan M. Demirel N, Uremis I. Insecticidal Effects of Some essential oils Against the Confused Flour Beetle (Tribolium confusum du Val) (Col.: Tenebrinoidea) in Stored Wheat. Asian Journal of Chemistry. 2009 ; 21(5) : 3995-4000.
5.    Mishra BB, Tripathi SP, Tripathi CPM. Research Article Investigation of Natural Plant Aegle marmelos Essential Oil Bioactivity on Development and Toxicity of Tribolium castaneum (Coleoptera: Tenebrionidae). International Journal of Zoological Research. 2016 ; 12 : 40-46.
6.    Liang JY, Wang WT, Zheng YF, Zhang D, Wang JL, Guo SS, Zhang WJ, Du SS, Zhang J. Bioactivities and Chemical Constituents of Essential Oil Extracted from Artemisia anethoides Against Two Stored Product Insects. J Oleo Sci. 2017; 66(1):71‐76.     
7.    Asgar E, Mohaddese M. Insecticidal activity of the essential oil isolated from Azilia eryngioides (PAU) hedge et lamond against two beetle pests. Chilean Journal of Agricultural Research. 2011; 71(3): 406-411.                                                  
8.    Mahmoudvand M., Abbasipour H., Basij M., Hossein Hosseinpour M., Rastegar F., Bagher Nasiri M. Fumigant toxicity of some essential oils on adults of some stored-product pests. Chilean Journal of Agricultural Research. 2011; 71(1):83-89.
9.    Mohammed M, Lahcen PE, Abderahmane K, Abdelmonaim HB. Insecticidal activity of the essential oil from seven accessions of Artemisia herba-alba Asso domesticated in Errachidia (south-east of Morocco) against Tribolium castaneum. Int Journal of Engineering Research and Applications. 2014; 4 (6):33-36.
10.    Hashemi SM, Safavi SA. Chemical constituents and toxicity of essential oils of oriental arborvitae, Platycladus orientalis (L.) Franco, against three stored-product beetles. Chilean Journal of Agricultural Research. 2012; 72(2): 188-194.
11.    Siddique S, Parveen Z, Butt A, Chaudhary MN, Akram M. Chemical Composition and Insecticidal Activities of Essential Oils of Myrtaceae against Tribolium castaneum (Coleoptera: Tenebrionidae). Polish Journal of Environmental Studies. 2017; 26(4): 1653-1662.
12.    Yan Y, Williams SB, Murdock LL, Baributsa D. Hermetic storage of wheat and maize flour protects against red flour beetle (Tribolium castaneum Herbst). PLoS ONE. 2017; 12(9): e0185386. DOI:
13.    Guo SS, Zhang W, Liang J, You C, Geng Z, Wang C, Du S. Contact and repellent activities of the essential oil from Juniperus formosana against two stored product insects. Molecules. 2016; 21(504): 1-11.
14.    Sharifian I, Hashemi SM, Aghaei M, Alizadeh M. Insecticidal activity of EO of Artemisia herba-alba Asso against three stored product beetles. Biharean Biologist. 2012; 6 (2): 90-93.
15.    Khleifat KM, Matar SA, Jaafreh M, Qaralleh H, Al-limoun MO, Alsharafa KY. Essential Oil of Centaurea damascena Aerial Parts, Antibacterial and Synergistic Effect. Journal of Essential Oil-Bearing Plants. 2019; 22(2): 356-367.
16.    Papachristos DP, Stamopoulos DC. Reppelent, toxic and reproduction inhibitory effects of essential oils vapors on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae. Journal of Stored Products Research. 2002 ; 38 (2) : 117-128.          
17.    Jilani G, Saxena RC. Repellent and feeding deterrent effects of turmeric oil, sweet flag oil, neem oil and a neem-based insecticide against lesser grain borer (Coleoptera, Bostrychidae). Journal of Economic Entomology. 1990; 83: 629-634.
18.    Nerio L.S., Olivero-Verbel J., Stashenko E. Repellent activity of essential oils: a review. Bioresource technology. 2010; 101(1): 372–378.
19.    Mc Donald LL, Guy RH, Speirs RD. 1970. Preliminary evaluation of new candidate materials as toxicants, repellents and attractants against stored product insects. Marketing Research Report. n° 882. Washington: Agricultural Research Service, United States Department of Agriculture. 183 pp.
20.    Novato TLP., Marchesini P, Muniz N, De Azevedo Prata MC, Furlong J, Vilela FMP, Daemon E, Maturano R, Monteiro C. Evaluation of synergism and development of a formulation with thymol, carvacrol and eugenol for Rhipicephalus microplus control. Experimental Parasitology. 2019; 207: 107774.
21.    Finney JD (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, UK
22.    Chou TC. Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacological reviews. 2006; 58: 621- 681.
23.    Novato TPL., Araújo LX, De Monteiro CMO, Maturano R, Senra TDOS, Da Silva Matos R, Daemon E. Evaluation of the combined effect of thymol, carvacrol and (E)-cinnamaldehyde on Amblyomma sculptum (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Veterinary parasitology. 2015; 212(3-4): 331-335.     
24.    Goudjil MB, Ladjel S, Bencheikh SE, Hammoya F, Bensaci MB, Zighmi S, Mehani M. Bioactivity of Artemisia Herba alba essential oil against plant pathogenic fungi. Der Pharma Chemica. 2016; 8(3):46-52.
25.    Lakehal S, Meliani A, Benmimoune S, Bensouna SN, Benrebiha FZ, et al. Essential Oil Composition and Antimicrobial Activity of Artemisia herba–alba Asso Grown in Algeria. Med chem (Los Angeles). 2016; 6: 435-439.                                        
26.    Bouzidi N, Mederbal K, Raho B. Antioxidant Activity of essential oil of Artemisia herba alba. Journal of Applied Environmental and Biological Sciences. 2016; 6(5): 59-65.
27.    Badreddine BS, Baouindi M. Effects of Artemisia herba-alba essential oils on survival stored cereal pests: Tribolium castaneum (Herbst) (Coleoptera, Tenebrionidae) and Trogoderma granarium (Everst) (Coleoptera, Dermestidae). Journal of Coastal Life Medicine. 2016; 4(5): 390-394.    
28.    Qnais EY, Alatshan AZ, Bseiso YG. Chemical composition, antinociceptive and anti-inflammatory effects of Artemisia herba-alba essential oil. J Food Agric Environ. 2016; 14: 20-27.
29.    Ouaritini ZB, Chraibi M, Benbrahim KF, Ez-zriouli R, Boukhira S, Darkaoui N. Phytochemical and antibacterial activity of the essential oil of Artemisia herba-alba from Morocco. IOSR Journal of Pharmacy and Biological Sciences. 2016; 11: 40-45.   
30.    Kadri A, Zarai Z, Chobba I. B, Békir A, Gharsallah N, Damak M, Gdoura R. Chemical constituents and antioxidant properties of Rosmarinus officinalis L. essential oil cultivated from South-Western Tunisia. J. Med. Plants Res. 2011; 5(29): 6502-6508.     
31.    Santoyo S, Cavero S, Jaime L, Ibanez E, Senorans FJ, Reglero G. Chemical composition and antimicrobial activity of Rosmarinus officinalis L. essential oil obtained via supercritical fluid extraction. Journal of food protection. 2005 ; 68(4) : 790-795.      
32.    Wang W, Li N, Luo M, Zu Y, Efferth T. Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules. 2012 ; 17(3) : 2704-2713.
33.    Ramdani M, Lograda T, Silini, H, Zeraib A, Chalard P, Figueredo G, Bouchaala M, Zerrar S. Antibacterial activity of essential oils of Juniperus phoenicea from Eastern Algeria. Journal of Applied Pharmaceutical Science. 2013; 3(11): 22-28.  
34.    El-Sawi S. A., Motawae HM, Ali AM. Chemical composition, cytotoxic activity and antimicrobial activity of essential oils of leaves and berries of Juniperus phoenicea L. grown in Egypt. African Journal of Traditional, Complementary and Alternative Medicines. 2007; 4(4): 417-426.
35.    Aljaiyash A, Ghanmi M, Satrani B, Labiad H, Echchelh A, Chaouch A. Chemical Composition of Essential Oils of Ripe and Unripe Berries and Leaves of Juniperus Phoenicea L. and determination of their Antimicrobial Activities. International Journal. 2016; 4(10): 7-14.
36.    Alfitori MO, Lamlom SH, Aly HM. Essential oil composition of leaves of Juniperus phoenicea grown at Al-Jabel Al-Akhdar Region, Libya. Middle East J Sci Res. 2014; 22: 368-370.
37.    Adams RP, Altarejos J, Arista Palmero M. Geographic variation in the volatile leaf oils J. phoenicea var. phoenicea from throughout its range. Phytologia. 2014; 96 (2):110-116.
38.    Liang JY, Guo SS, Zhang WJ, Geng ZF, Deng ZW, Du SS, Zhang J. Fumigant and repellent activities of essential oil extracted from Artemisia dubia and its main compounds against two stored product pests. Natural Product Research. 2018; 32(10):1234-1238.
39.    Hashemi S. M., Rostaefar A. Insecticidal Activity of Essential Oil from Juniperus communis L. subsp. hemisphaerica (Presl) Nyman against Two Stored Product Beetles. Ecologia Balkanica. 2014; 6(1): 87-93.
40.    Guo SS, You CX, Liang JY, Zhang WJ, Geng ZF, Wang CF, Lei N. Chemical composition and bioactivities of the essential oil from Etlingera yunnanensis against two stored product insects. Molecules. 2015; 20(9) :15735-15747.
41.    Zarrad K, Hamouda A. B, Chaieb I, Laarif A, Jemâa JMB. Chemical composition, fumigant and anti-acetylcholinesterase activity of the Tunisian Citrus aurantium L. essential oils. Industrial Crops and Products. 2015; 76: 121-127.
42.    Bedini S, Bougherra HH, Flamini G, Cosci F, Belhamel K, Ascrizzi R, Conti B. Repellency of anethole-and estragole-type fennel essential oils against stored grain pests: the different twins. Bull Insectol. 2016; 69(1): 149-157.
43.    Zibaee I, Khorram P. Synergistic effect of some essential oils on toxicity and knockdown effects, against mosquitos, cockroaches and housefly. Arthropods. 2015; 4(4): 107-123.
44.    Tong F, Bloomquist JR. Plant essential oils affect the toxicities of carbaryl and permethrin against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology. 2013; 50(4): 826-832.
45.    Araújo LX, Novato TPL, Zeringota V, Maturano R, Melo D, Da Silva BC, Daemon OE, De Carvalho M.G, Monteiro CM. Synergism of thymol, carvacrol and eugenol in larvae of the cattle tick, Rhipicephalus microplus, and brown dog tick, Rhipicephalus sanguineus. Medical and veterinary entomology. 2016; 30(4) 377-382.
46.    Enan E. Insecticidal activity of essential oils: octopaminergic sites of action. Comp Biochem Physiol C Toxicol Pharmacol. 2001; 130(3):325‐337.
47.    Fatiha RA, Kada R, Khelil MA, Pujade-Villar J. Biological control against the cowpea weevil (Callosobruchus chinensis L., Coleoptera: Bruchidae) using essential oils of some medicinal plants. Journal of Plant Protection Research. 2014; 54 (3): 211-217.
48.    Olmedo R, Herrera JM, Lucini EI, Zunino MP, Pizzolitto RP, Dambolena JS, Zygadlo JA. Essential oil of Tagetes filifolia against the flour beetle Tribolium castaneum and its relation to acetylcholinesterase activity and lipid peroxidation. Agriscientia. 2015; 32(2): 113-121.
49.    Douiri LF, Boughdad A, Alaoui MH, Mohammed M, Morocco M. Biological activity of Rosmarinus officinalis essential oils against Callosobruchus maculatus, (Coleoptera, Bruchinae). Journal of Biology, Agriculture and Healthcare. 2014; 4(2) 5-14.
50.    Faraone N, Hillier NK, Cutler GC. 2015. Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae). PLoS ONE 10(5): e0127774. DOI :
51.    Rosato A, Carocci A, Catalano A, Clodoveo ML, Franchini C, Corbo F, Carbonara GG, Carrieri A, Fracchiolla G. Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials. PLoS ONE. 2018; 13(8): e0200902. DOI:

Recomonded Articles:

Author(s): Jagadeesh Narkedimilli, Vishweshwar Vavilala, Sandeep Mohanty, Saravanan Manvalan, Jayashree Anireddy

DOI: 10.5958/0974-4150.2018.00096.2         Access: Open Access Read More

Author(s): Anupam Maurya, Subash C. Verma, Vijay Gupta, M. B. Shankar

DOI: 10.5958/0974-4150.2017.00142.0         Access: Open Access Read More

Author(s): Cijo John, Mohd Idris, P. Sudhakar, Priyankar Ghosh, K.M. Varshney, S.K. Shukla

DOI:         Access: Open Access Read More

Author(s): M. Yogeshwar Reddy, V. Ramesh, Ch. Kista Reddy, N. Venugopal, G. Saravanana, M.V. Suryanarayana, B. Shyam Sunder , R.Surendranath Reddy, G. Raju

DOI:         Access: Open Access Read More

Author(s): Yogeshwar Reddy M, Ramesh V, Kista Reddy Ch, Suryanarayana M.V, Dilip Kumar M, Raju G, Saravanan G, Debashish Datta

DOI:         Access: Open Access Read More

Author(s): D Kilimozhi, V Parthasarathy, R Manavalan

DOI:         Access: Open Access Read More

Author(s): Archana Kulkarni, Nasreen Jan, Seema Nimbarte

DOI:         Access: Open Access Read More

Author(s): Rajabudeen E., Saravana Ganthi A., M. Padma Sorna Subramanian

DOI:         Access: Open Access Read More

Author(s): G. Raveendra Babu, M. Rama Ayyappa, B. Krishna, A. Ravi Kumar, G.L.S. Priyanaka, P.N.V.S.S. Pavani, CH. L.Prasanna, B. Anjil, G.Harish

DOI: 10.5958/0974-4150.2019.00050.6         Access: Open Access Read More

Author(s): Soram Ibomcha Singh, Shashi Prabha

DOI:         Access: Open Access Read More

Author(s): U.S. Pujeri, S.C. Hiremath, A.S. Pujari, K.G. Pujari, M.I. Kumbar, M.S. Yadawe

DOI:         Access: Open Access Read More

Author(s): A. Anandan, R. Eswaran, A.Doss, G. Sangeetha, S. P. Anand

DOI:         Access: Open Access Read More

Author(s): Dwivedi Abha, Kaushik Avinash, Sharma Ganesh N., Akhtar Javed

DOI:         Access: Open Access Read More

Author(s): Mahesh K. Saini, Sudeep Mishra, Samsul Alam, Lalitesh K. Thakur, Ompal Singh, Syed K. Raza

DOI: 10.5958/0974-4150.2016.00003.1         Access: Open Access Read More

Author(s): Yogeshwar Reddy M*, Ramesh V, Kista Reddy Ch, Venugopal N, Saravanan G, Suresh Y, Suryanarayana M, Debashish Datta, Raju B

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles

Recent Articles