Author(s):
Omar Mechraoui, Ali Imessaoudene, Mohamed Y. Maiz, Hicham Banouh, Lotfi Mouni, Abdelkrim Rebiai, Mohamed L. Belfar, Noureddine Elboughdiri, Djamel Ghernaout, Bachir Ben Seghir
Email(s):
bbachir39@gmail.com
DOI:
10.52711/0974-4150.2021.00069 
Address:
Omar Mechraoui1, Ali Imessaoudene2, Mohamed Y. Maiz2, Hicham Banouh2, Lotfi Mouni2, Abdelkrim Rebiai3, Mohamed L. Belfar4, Noureddine Elboughdiri5,6, Djamel Ghernaout5,7, Bachir Ben Seghir8,9,10*
1Faculty of Technology, University Amar Telidji-Laghouat, P.O. Box 37G, Laghouat 03000, Algeria.
2Laboratory of Management and Valorization of Natural Resources and Quality Assurance, SNVST Faculty, University of Bouira, Bouira 10000, Algeria.
3Chemistry Department, Faculty of Exact Sciences, University of El Oued, P.O. Box 789, El Oued 39000, Algeria.
4Department of Chemistry, Faculty of Mathematics and Material Science, University Kasdi Merbah Ouargla, P.O. Box 511, Ouargla 30000, Algeria.
5Chemical Engineering Department, College of Engineering, University of Ha’il, P.O. Box 2440, Ha'il 81441, Saudi Arabia.
6Chemical Engineering Process Department, National School of Engineering Gabes, University of Gabes, Gabes 6011, Tunisia.
7Chemical Engineering Department, Faculty of Engin
Published In:
Volume - 14,
Issue - 6,
Year - 2021
ABSTRACT:
Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620nm using UV/Vis spectrophotometer. Total phenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract (5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract that is estimated at 0.18±0.01 mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibited calcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones. Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620 nm using UV/Vis spectrophotometer. Total phenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract (5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract that is estimated at 0.18±0.01mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibited calcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones.
Cite this article:
Omar Mechraoui, Ali Imessaoudene, Mohamed Y. Maiz, Hicham Banouh, Lotfi Mouni, Abdelkrim Rebiai, Mohamed L. Belfar, Noureddine Elboughdiri, Djamel Ghernaout, Bachir Ben Seghir. In vitro Antiurolithiatic activity of the leaves and flowers extracts of Paronychia argentea, a plant used in traditional medicine in Algeria. Asian Journal of Research in Chemistry. 2021; 14(6):401-1. doi: 10.52711/0974-4150.2021.00069
Cite(Electronic):
Omar Mechraoui, Ali Imessaoudene, Mohamed Y. Maiz, Hicham Banouh, Lotfi Mouni, Abdelkrim Rebiai, Mohamed L. Belfar, Noureddine Elboughdiri, Djamel Ghernaout, Bachir Ben Seghir. In vitro Antiurolithiatic activity of the leaves and flowers extracts of Paronychia argentea, a plant used in traditional medicine in Algeria. Asian Journal of Research in Chemistry. 2021; 14(6):401-1. doi: 10.52711/0974-4150.2021.00069 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2021-14-6-2
REFERENCES:
1. Winkler C. Di Cocco G. Gehrels N. Giménez A. Grebenev S. Hermsen W. et al. The integral mission. Astronomy & Astrophysics. 2003;411:L1-L6. https://doi.org/10.1051/0004-6361:20031288
2. Daudon M. Bounxouei B. Santa Cruz F. Leite Da Silva S. Diouf B. Angwafoo III F. Talati J. Desrez G. Composition des calculs observés aujourd’hui dans les pays non industrialisés. Prog Urol. 2004;14:1151-1161.
3. Charafi F. Apports des techniques moléculaires en bactériologie; 2012. http://ao.um5s.ac.ma/xmlui/handle/123456789/1800
4. Afifi TD. Olson LN. Armstrong C. The chilling effect and family secrets: Examining the role of self protection, other protection, and communication efficacy. Human Communication Research. 2005;31:564-598. https://doi.org/10.1111/j.1468-2958.2005.tb00883.x
5. Beloued A. Medicinal plants of Algeria. Alger: Office of University Publications 1998:62.
6. Zama D. Meraihi Z. Tebibel S. Benayssa W. Benayache F. Benayache S. et al. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian Journal of Pharmacology. 2007;39:145. DOI: 10.4103/0253-7613.33434
7. Al-Bakri AG. Afifi FU. Evaluation of antimicrobial activity of selected plant extracts by rapid XTT colorimetry and bacterial enumeration. Journal of Microbiological Methods. 2007;68:19-25. DOI: 10.1016/j.mimet.2006.05.013
8. Ferreira A. Proença C. Serralheiro M. Araujo M. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. Journal of ethnopharmacology. 2006;108:31-37. DOI: 10.1016/j.jep.2006.04.010
9. Bouanani S. Henchiri C. Migianu-Griffoni E. Aouf N. Lecouvey M. Pharmacological and toxicological effects of Paronychia argentea in experimental calcium oxalate nephrolithiasis in rats. Journal of ethnopharmacology. 2010;129:38-45. DOI: 10.1016/j.jep.2010.01.056
10. Dafni A. Mimicry and deception in pollination. Annual review of ecology and systematics. 1984;15:259-278. https://doi.org/10.1146/annurev.es.15.110184.001355
11. Braca A. Siciliano T. D’Arrigo M. Germanò MP. Chemical composition and antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia. 2008,;79:123-125. DOI: 10.1016/j.fitote.2007.11.002
12. Georgé S. Brat P. Alter P. Amiot MJ. Rapid determination of polyphenols and vitamin C in plant-derived products. Journal of Agricultural and food chemistry. 2005;53:1370-1373. DOI: 10.1021/jf048396b
13. Djeridane A. Yousfi M. Nadjemi B. Boutassouna D. Stocker P. Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food chemistry. 2006;97:654-660. https://doi.org/10.1016/j.foodchem.2005.04.028
14. Sasikala V. Radha SR. Vijayakumari B. In vitro evaluation of Rotula aquatica Lour. for antiurolithiatic activity. Journal of Pharmacy Research. 2013;6:378-382. DOI: 10.1016/j.jopr.2013.02.026
15. Bouabdelli F. Djelloul A. Kaid-Omar Z. Semmoud A. Addou A. Antimicrobial activity of 22 plants used in urolithiasis medicine in Western Algeria. Asian Pacific Journal of Tropical Disease. 2012;2:S530-S535. https://doi.org/10.1016/S2222-1808(12)60215-
16. Adjadj M. Baghiani A. Boumerfeg S. Noureddine C. Khennouf S. Arrar L. et al. Protective effect of Paronychia argentea L. on acetic acid induced ulcerative colitis in mice by regulating antioxidant parameters and inflammatory markers. Der. Pharma. Chemica. 2016;8:207-218.
17. Wang J-X. Xiao X-H. Li G-K. Study of vacuum microwave-assisted extraction of polyphenolic compounds and pigment from Chinese herbs. Journal of Chromatography A. 2008;1198:45-53. DOI: 10.1016/j.chroma.2008.05.045
18. Li H. Deng Z. Wu T. Liu R. Loewen S. Tsao R. Microwave-assisted extraction of phenolics with maximal antioxidant activities in tomatoes. Food chemistry. 2012;130:928-936. DOI: 10.3390/molecules23102498
19. Bonnaillie C. Salacs M. Vassiliova E. Saykova I. Etude de l’extraction de composés phénoliques à partir de pellicules d’arachide (Arachis hypogaea L.). Revue Nature et Technologie. 2012;5:35-40
20. Bruneton J. Pharmacognosie, phytochimie, plantes médicinales. Lavoisier. 1999.
21. Green RJ. Antioxidant activity of peanut plant tissues. 2004. http://www.lib.ncsu.edu/resolver/1840.16/371
22. Do QD. Angkawijaya AE. Tran-Nguyen PL. Huynh LH. Soetaredjo FE. Ismadji S. Yi-Hsu J. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of food and drug analysis. 2014;22:296-302. DOI: 10.1016/j.jfda.2013.11.001
23. Macheix J-J. Fleuriet A. Jay-Allemand C. Les composés phénoliques des végétaux: un exemple de métabolites secondaires d'importance économique: PPUR presses polytechniques; 2005.
24. Zeng X. Xi Y. Jiang W. Protective roles of flavonoids and flavonoid-rich plant extracts against urolithiasis: a review. Critical reviews in food science and nutrition. 2019;59:2125-2135. DOI: 10.1080/10408398.2018.1439880
25. Contini M. Baccelloni S. Massantini R. Anelli G. Extraction of natural antioxidants from hazelnut (Corylus avellana L.) shell and skin wastes by long maceration at room temperature. Food chemistry. 2008;110:659-669. DOI: 10.1155/2018/7470384
26. Ammor K. Bousta D. Jennan S. Bennani B. Chaqroune A. Mahjoubi F. Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco. The Scientific World Journal. 2018; 7 https://doi.org/10.1155/2018/74703842018
27. Aganga A. Mosase K. Tannin content, nutritive value and dry matter digestibility of Lonchocarpus capassa, Zizyphus mucronata, Sclerocarya birrea, Kirkia acuminata and Rhus lancea seeds. Animal Feed Science and Technology. 2001;91:107-113. DOI: 10.1016/s0377-8401(01)00235-8
28. Pedneault K. Leonharts A. Gosselin A. Ramputh A. Arnason J. Influence de la culture hydroponique de quelques plantes médicinales sur la croissance et la concentration en composés secondaires des organes végétaux. In: Texte de conférence. Canada; 2001;1-5.
29. Fiorucci S. Activités biologiques de composés de la famille des flavonoïdes: Approches par des méthodes de chimie quantique et de dynamique moléculaire: Université de Nice Sophia-Antipolis (UNS); 2006.
30. Pawlowska AM. Camangi F. Braca A. Quali-quantitative analysis of flavonoids of Cornus mas L.(Cornaceae) fruits. Food chemistry. 2010;119:1257-1261. http://eprints.adm.unipi.it/id/eprint/656
31. Rajeshwari P. Rajeswari G. Jabbirulla S. Vishnu Vardhan I. Evaluation of in vitro antiurolithiasis activity of Convolvulus arvensis. Int J Pharm Pharm Sci. 2013;5:599-601.
32. Beghalia M. Ghalem S. Allali H. Belouatek A. Marouf A. Inhibition of calcium oxalate monohydrate crystal growth using Algerian medicinal plants. Journal of Medicinal Plants Research. 2008;2:066-070. https://doi.org/10.5897/JMPR.9000798
33. Coe KL-PF. Fenech GL-PM. 11th International Symposium on Urolithiasis, Nice, France, 2–5 September 2008. Urol Res. 2008;36:157-232.
34. Joshi V. Parekh B. Joshi M. Vaidya A. Herbal extracts of Tribulus terrestris and Bergenia ligulata inhibit growth of calcium oxalate monohydrate crystals in vitro. Journal of Crystal Growth. 2005;,275:e1403-e1408. https://doi.org/10.1016/j.jcrysgro.2004.11.240
35. Suzuki K. Kawamura K. Tsugawa R. Formation and growth inhibition of calcium oxalate crystals by takusha (alismatis rhizoma). Scanning microscopy. 1999;13:183-189.