Author(s):
Harishchandra Jirimali, Saiprasad Santre, Shubhangi Deonikar, Bhausaheb More, Sanjay Mhaske
Email(s):
hdj739@gmail.com , bhaumore1@gmail.com
DOI:
10.52711/0974-4150.2026.00018 
Address:
Harishchandra Jirimali1*, Saiprasad Santre1,2, Shubhangi Deonikar1, Bhausaheb More1*, Sanjay Mhaske3
1Regional Forensic Science Laboratory, Nanded (MS) India. 431601
2Department of Chemistry, Yeshwant Mahavidyalaya, Nanded (MS) India. 431602
3Department of Chemistry, Wilson College, Mumbai (MS) India. 400007
*Corresponding Author
Published In:
Volume - 19,
Issue - 2,
Year - 2026
ABSTRACT:
A new chromogenic spray reagent is described for chromatographic detection and identification of bromadiolone, a second-generation anticoagulant rodenticide, by TLC. The reagent is prepared by simple diazotization of o-Tolidine by using hydrochloric acid and sodium nitrite. The standard bromadiolone was spotted on TLC (thin layer chromatography) and developed in Chloroform: Acetone and 1mL Acetic acid (glacial). The prepared reagent is sprayed on the TLC, forming reddish-pink colour adduct with bromadiolone. This method showed selective detection of bromadiolone in the presence of other interfering pesticides; also, it shows reproducibility. The possible mechanism suggests the formation of a diazotized adduct of the reagent with bromadiolone, which shows a stable reddish-pink coloured spot. This method is very useful for the detection of the bromadiolone in the forensically important cases like suicides where it is important to detect the bromadiolone selectively in the presence of other pesticides which could be also used in suicidal or homicidal cases.
Cite this article:
Harishchandra Jirimali, Saiprasad Santre, Shubhangi Deonikar, Bhausaheb More, Sanjay Mhaske. A New Chromogenic Spray Reagent for Detection and Identification of Bromadiolone in Biological Samples. Asian Journal of Research in Chemistry.2026; 19(2):109-2. doi: 10.52711/0974-4150.2026.00018
Cite(Electronic):
Harishchandra Jirimali, Saiprasad Santre, Shubhangi Deonikar, Bhausaheb More, Sanjay Mhaske. A New Chromogenic Spray Reagent for Detection and Identification of Bromadiolone in Biological Samples. Asian Journal of Research in Chemistry.2026; 19(2):109-2. doi: 10.52711/0974-4150.2026.00018 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2026-19-2-5
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