ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Tripti Jain, Amber Vyas, Darshan Dubey, Kamlesh Dashora, Vishal Jain

Email(s): vishaljain123@gmail.com

DOI: 10.52711/0974-4150.2022.00058   

Address: Tripti Jain1, Amber Vyas2, Darshan Dubey3, Kamlesh Dashora3, Vishal Jain2*
1Chhattisgarh Food and Drug Administration, Mahasamund (CG).
2University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.).
3Institute of Pharmacy, Vikram University, Ujjain (MP).
*Corresponding Author

Published In:   Volume - 15,      Issue - 5,     Year - 2022


ABSTRACT:
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.


Cite this article:
Tripti Jain, Amber Vyas, Darshan Dubey, Kamlesh Dashora, Vishal Jain. Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach. Asian Journal of Research in Chemistry. 2022; 15(5):327-0. doi: 10.52711/0974-4150.2022.00058

Cite(Electronic):
Tripti Jain, Amber Vyas, Darshan Dubey, Kamlesh Dashora, Vishal Jain. Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach. Asian Journal of Research in Chemistry. 2022; 15(5):327-0. doi: 10.52711/0974-4150.2022.00058   Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2022-15-5-4


REFERENCES:
1.    Jain V., Saraf S. and Saraf S. Spectrophotometric determination of Piperine in Trikatu Churna: An Ayurvedic Formulation, Asian Journal of Chemistry. 2007, 19, (7), 5331-5335.
2.    World Health Organization, Quality Control Methods for Medicinal Plants Materials, Geneva, 1998, 1-15.
3.    WHO general guidelines for methodologies on research and evaluation of traditional medicine. (2000): http://whqlibdoc.who.int/hq/2000/WHO_EDM_TRM_2000.1.pdf.
4.    Gupta M., Chaudhary P.H., Mukund G. Shrivastava T.B., Need and scope of standardization of herbal medicines - A review, International Journal of Green Pharmacy. Oct-Dec 2021 ,16 (1), 346-352.
5.    Formulary of Siddha Medicine. 4th ed., Published by Indian Medical Practitioners Cooperative Pharmacy and Store Ltd (IMPCOPS), Chennai, 1993: 45.
6.    Indian Herbal Pharmacopoeia, Regional Research Laboratory Jammu, Indian drug Manufacturing Association Mumbai. 1999.
7.    Gurinderdeep S, Piperine: A Remarkable Marker with Intense Biological Activity, International Journal of Pharmacognosy and Chinese Medicine. 2017, 1(4): 122.

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